FOXM1 anticorps (C-Term)

N° du produit ABIN490076

L’anticorps Lapin Polyclonal anti-FOXM1 a été validé pour WB, IHC et IHC (p). Il convient pour détecter FOXM1 dans des échantillons de Humain, Chien, Cobaye et Porc.

Aperçu rapide pour FOXM1 anticorps (C-Term) (ABIN490076)

Antigène: FOXM1 (Forkhead Box M1 (FOXM1))

Reactivité: Humain, Chien, Cobaye, Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FOXM1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-FOXM1 anticorps

Épitope: C-Term

Marque: IHC-plus™

Specificité: Human FOXM1

Homologie: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Rabbit (91%) Dog, Pig, Guinea pig (83%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide from C-Terminus of human FOXM1 (Q08050, NP_068772). Percent identity by BLAST analysis: Human, Chimpanzee (100%), Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Rabbit (91%), Elephant (90%), Galago, Panda, Dog, Pig, Guinea pig (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur FOXM1

Antigène: FOXM1 (Forkhead Box M1 (FOXM1))

Autre désignation: FOXM1

Sujet: Name/Gene ID: FOXM1
Family: Transcription factor

Synonyms: FOXM1, Forkhead box M1, Forkhead box protein M1, HFH11, HNF-3, HNF-3/fork-head homolog 11, INS-1, FOXM1B, M-phase phosphoprotein 2, MPP-2, MPHOSPH2, PIG29, TRIDENT, FKHL16, HFH-11, TGT3, Transcription factor Trident, WIN

ID gène: 2305

NCBI Accession: NP_068772

UniProt: Q08050

Pathways: Positive Regulation of Response to DNA Damage Stimulus