KIF20A anticorps (C-Term)

N° du produit ABIN6744463

Cet anticorps anti-KIF20A est un anticorps Lapin Polyclonal détectant KIF20A dans WB, IHC et IHC (p). Adapté pour Humain, Rat, Chien, Cheval, Porc, Lapin et Boeuf (Vache).

Aperçu rapide pour KIF20A anticorps (C-Term) (ABIN6744463)

Antigène: KIF20A (Kinesin Family Member 20A (KIF20A))

Reactivité: Humain, Rat, Chien, Cheval, Porc, Lapin, Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp KIF20A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-KIF20A anticorps

Épitope: C-Term

Marque: IHC-plus™

Specificité: Human KIF20A

Homologie: Percent identity by BLAST analysis: Human, Dog, Rabbit, Horse, Pig (100%) Chicken (92%) Rat (86%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide from C-Terminus of human KIF20A (O95235, NP_005724). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Opossum (100%), Galago, Marmoset, Mouse, Bat, Guinea pig (93%), Turkey, Chicken (92%), Rat (86%), Zebra finch (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur KIF20A

Antigène: KIF20A (Kinesin Family Member 20A (KIF20A))

Autre désignation: RAB6KIFL / KIF20A

Sujet: Name/Gene ID: KIF20A

Synonyms: KIF20A, Kinesin family member 20A, Kinesin-like protein KIF20A, GG10_2, Mitotic kinesin-like protein 2, MKLP2, Rabkinesin-6, RAB6KIFL

ID gène: 10112

NCBI Accession: NP_005724

UniProt: O95235