CPXCR1 anticorps (AA 396-445)

N° du produit ABIN6744465

L’anticorps Lapin Polyclonal anti-CPXCR1 a été validé pour WB, IHC et IHC (p). Il convient pour détecter CPXCR1 dans des échantillons de Humain.

Aperçu rapide pour CPXCR1 anticorps (AA 396-445) (ABIN6744465)

Antigène: CPXCR1 (CPX Chromosome Region, Candidate 1 (CPXCR1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CPXCR1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-CPXCR1 anticorps

Épitope: AA 396-445

Marque: IHC-plus™

Specificité: Human CPXCR1

Homologie: Percent identity by BLAST analysis: Human (100%) Monkey (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa396-445 of human CPXCR1 (Q8N123, NP_149037). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Gibbon, Monkey (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (10 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur CPXCR1

Antigène: CPXCR1 (CPX Chromosome Region, Candidate 1 (CPXCR1))

Autre désignation: CPXCR1 / CT77

Sujet: Name/Gene ID: CPXCR1

Synonyms: CPXCR1, Cancer/testis antigen 77, CT77

ID gène: 53336

NCBI Accession: NP_149037

UniProt: Q8N123