Sialylated Mucin-Type-O-Glycans anticorps

N° du produit ABIN6757949

Cet anticorps anti- est un anticorps Souris Monoclonal détectant dans IHC (p), ELISA, IHC et ICC. Adapté pour Humain.

Aperçu rapide pour Sialylated Mucin-Type-O-Glycans anticorps (ABIN6757949)

Antigène: Sialylated Mucin-Type-O-Glycans

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: FW17

Détail du produit

Specificité: Sialylated Mucin-Type-O-Glycans.

Purification: Purified

Immunogène: Purified mucus-glycoproteins from human amniotic fluid.

Isotype: IgM

Information d'application

Indications d'application: Approved: ELISA, ICC, IHC, IHC-P

Usage: Tumor and normal tissues were first fixed in 6 % neutral phosphate-buffered formalin and dehydrated in ethanol by standard histological techniques. After embedding all tissues in paraffin, serial microtome tissue sections (5 μm) were used for comparative immunohistological studies. Reactivity of hybridoma tissue culture supernatants was determined using a four-step immunoperoxidase technique. After deparaffinization in xylene, rehydration through graded ethanol, and blocking endogenous peroxidase activity with 2 % H202 in absolute methanol for 30 min, the sections were incubated as follows: (1) normal swine serum (5 % in PBS), (2) MAb in tissue culture supernatant or mouse ascites fluid diluted to 1/100 in PBS, (3) rabbit anti-mouse immunoglobulin (2 % in PBS), (4) swine anti-rabbit immunoglobulin (2 % in PBS), (5) rabbit peroxidase anti-peroxidase (PAP) complex. The slides were treated with 0.2 mg/mL - ' 3-amino-9-ethylcarbazole in 0.02 M sodium acetate buffer containing 0.01 % H202, then counterstained with hematoxylin, and mounted in glycerol jelly. All incubation steps were performed in a moist chamber at 21 °C for 30 min.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire: Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.

Détail du antigène

Antigène: Sialylated Mucin-Type-O-Glycans