IL-25 anticorps
Aperçu rapide pour IL-25 anticorps (ABIN6774203)
Antigène
Voir toutes IL-25 (IL25) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- Recognizes mouse IL-17E. In sandwich ELISAs no cross-reactivity or interference was observed with rhIL-17E, rmIL-17, rmIL-17B, C, D, or F.
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Purification
- Protein G purified
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Immunogène
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E. coli-derived rmIL-17E.
Type of Immunogen: Recombinant protein -
Isotype
- IgG2b
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Indications d'application
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Approved: ELISA, Flo
Usage: Suitable for use in ELISA and Flow Cytometry. ELISA (Capture): 2-8 μg/m. Can be used as a capture reagent in a sandwich immunoassay in combination with a biotinylated, mouse IL-17E antibody and recombinant mouse IL-17E, as the standard. Flow Cytometry: For intracellular flow cytometry to detect IL-17E, cells must first be fixed and permeabilized with 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled antibody may be visualized by adding a secondary developing reagent such as anti-rat IgG conjugated to a fluorochrome. -
Commentaires
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Target Species of Antibody: Mouse
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- 40-50 % glycerol, PBS.
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Concentration
- Lot specific
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Buffer
- Lyophilized from PBS, 5 % trehalose.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- IL-25 (IL25) (Interleukin 25 (IL25))
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Autre désignation
- IL25 / IL17E
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Sujet
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Name/Gene ID: IL25
Family: Interleukin
Synonyms: IL25, Interleukin 17E, Interleukin 25, Interleukin-25, IL17E, IL-17E, IL-25, Interleukin-17E -
ID gène
- 64806
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UniProt
- Q9H293
Antigène
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