Msk1/2 (AA 49-94) anticorps (Cy7)

N° du produit ABIN682624

Détail du produit

Antigène: Msk1/2

Épitope: AA 49-94

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cy7

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Réactivité croisée: Humain

Homologie: Mouse,Rat,Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human MSK1

Isotype: IgG

Information d'application

Indications d'application: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: Msk1/2

Sujet:

Synonyms: MSK1, RPS6KA5, 90 kDa ribosomal protein S6 kinase 5, EC 2.7.11.1, KS6A5_HUMAN, MGC1911, Mitogen and stress activated protein kinase 1, MSPK1, Nuclear Mitogen And Stress Activated Protein Kinase 1, Nuclear mitogen- and stress-activated protein kinase 1, Ribosomal protein S6 kinase 90kD polypeptide 5, Ribosomal protein S6 kinase 90 kDa, Ribosomal protein S6 kinase 90 kDa polypeptide 5, Ribosomal Protein S6 Kinase Alpha 5, Ribosomal protein S6 kinase alpha-5, RLPK, RSK Like Protein Kinase, RSK-like protein kinase, RSKL, S6K alpha 5, S6K-alpha-5, RPS6KA4,S6K-alpha-4, 90 kDa ribosomal protein S6 kinase 4, Ribosomal protein kinase B, RSKB.

Background: MSK1 is a mitogen and stress activated protein kinase 1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK1 can be activated by ERK1/2 and SAPK2/p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG14 in response to mitogen and stress. Similar to RSK, MSK1 contains two kinase domains (N term and a C term). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C terminal kinase domain of MSK1 which is essential for the catalytic activity of the N terminal kinase domain. MSK2 plays an essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors.

ID gène: 9252, 8986