DAB1 anticorps (pSer524) (Cy5)

N° du produit ABIN684362

L’anticorps Lapin Polyclonal anti-DAB1 a été validé pour WB, IF (cc) et IF (p). Il convient pour détecter DAB1 dans des échantillons de Humain et Souris.

Aperçu rapide pour DAB1 anticorps (pSer524) (Cy5) (ABIN684362)

Antigène: DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DAB1 est conjugé à/à la Cy5

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Détail du produit anti-DAB1 anticorps (Cy5)

Épitope: pSer524

Réactivité croisée: Humain, Souris

Homologie: Rat,Dog,Cow,Sheep,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic phosphopeptide derived from mouse Dab1 around the phosphorylation site of Ser524

Isotype: IgG

Information d'application

Indications d'application: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur DAB1

Antigène: DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))

Autre désignation: Dab1

Sujet:

Synonyms: Dab 1, Disabled homolog 1, Disabled homolog 1 Drosophila, Scm, Scr, Scrambler, Yot, Yotari.

Background: Disabled 1 (Dab1) is an 80 kDa protein that is encoded by the Disabled-1 gene locus which is mutated in scrambler and yotari mutant mice. Phenotypically, the mutation of this gene produces motor defects and ataxia, disruption of neuronal migration, and severe cerebellar hypoplasia. Dab1 is an intracellular adapter protein that functions in downstream signaling events initiated by the secreted protein reelin. Dab1 contains a phosphotyrosine binding (PTB) domain in the amino terminus. Tyrosine phosphorylation of Dab1 is increased by reelin binding to the Very Low Density Lipoprotein Receptor (VLDLR) and Apolipoprotein E Receptor 2 (ApoER2) through stimulation of Src family kinases. Src family kinase and c-Abl activities are themselves then stimulated by binding to tyrosine phosphorylated Dab1. Dab1 also mediates activation of Akt (PKB) by reelin resulting in inhibition of glycogen synthase kinase 3 beta (GSK-3 beta) and decreased phosphorylation of the microtubule-associated protein, Tau. Dab1 serine 491 is phosphorylated in a Cdk5-dependent manner and regulates, likely indirectly, Reelin-induced signaling during neural cortex development.