Cadherin anticorps (AA 841-882)

N° du produit ABIN687682

Cet validé indépendamment anticorps Lapin Polyclonal détecte spécifiquement Cadherin dans WB, FACS, IF (cc), IF (p), IHC (p), ELISA, IHC (fro) et ICC. Il présente une réactivité envers Humain et Souris et a été mentionné dans 2+ publications.

Aperçu rapide pour Cadherin anticorps (AA 841-882) (ABIN687682)

Antigène: Cadherin

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cadherin est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Détail du produit anti-Cadherin anticorps

Épitope: AA 841-882

Réactivité croisée: Humain, Souris

Homologie: Rat,Dog,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human E-cadherin

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Independent Validation (FACS)

Validation #029742 (Flow Cytometry)

by: Flow Cytometry & Cell Separation Facility, Purdue University

No.: #029742

Date: 30.06.2014

Numéro du lot: 131101

Application validée: Flow Cytometry

Contrôle positif: MCF7 cells (high expression)

Contrôle négative: SH-SY5Y cells (low expression)

Conclusion: Signal was observed in the positive control cells. There was some non-specific staining observed with the isotype and secondary-only antibody controls which was lower than the signal from the anti-E-cadherin antibody. Low signal was observed in the low expressing (negative control) cells as expected.

Validation Images

Figure 1. Histogram of MCF7 cells stained with anti-E-cadherin (orange), isotype control antibody (green), secondary antibody only (blue) and unstained (red).

Figure 2. Histogram of SH-SY5Y cells stained with anti-E-cadherin (orange), isotype control antibody (green), secondary antibody only (blue) and unstained (red).

Protocole

Anticorps primaire:

• Antigen: Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1)
• Catalog number: ABIN687682
• Lot number: 131101

Anticorps secondaire:

• Antibody: Goat anti-rabbit IgG-Alexa 647

Full Protocol:

• Positive and negative control cells were washed once with phosphate-buffered saline (PBS) and harvested with a non-enzymatic cell dissociation solution (Cellstripper, Mediatech, Inc).
• Detached cells were washed twice and resuspended in 100 µL 1X PBS containing 0.5% BSA: - unstained cells - secondary antibody alone - 1 µg primary antibody - 1 µg isotype control antibody
• Cells were incubated for 30 min on ice.
• Labeled cells were washed twice in PBS containing 0.5% BSA.
• Cells were resuspended with 1X PBS containing 0.5% BSA + 10% goat serum and incubated for 15 min at room temperature.
• Goat anti-rabbit IgG-Alexa 647 secondary antibody (Jackson Immunoresearch) was added at a 1:500 dilution. The cells were incubated for 30 min in the dark on ice.
• Labeled cells were washed twice in PBS containing 0.5% BSA.
• Propidium Iodide (PI) was added to discern live cells from dead cells.
• Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).

Notes: - The data displayed is gated on PI negative cells.

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-Cadherin (ABIN687682)

Yu, Wang, Tang, Zhang, Shang: "RNA interference-mediated knockdown of Notch-1 inhibits migration and invasion, down-regulates matrix metalloproteinases and suppresses NF-?B signaling pathway in trophoblast cells." dans: Acta histochemica, Vol. 116, Issue 5, pp. 911-9, (2014) (PubMed).

Fan, Wang, Zhao, Liu, Chen, Sun, Zheng: "Sonic hedgehog signaling may promote invasion and metastasis of oral squamous cell carcinoma by activating MMP-9 and E-cadherin expression." dans: Medical oncology (Northwood, London, England), Vol. 31, Issue 7, pp. 41, (2014) (PubMed).

Détails sur Cadherin

Antigène: Cadherin

Sujet:

Synonyms: UVO, CDHE, ECAD, LCAM, Arc-1, CD324, Cadherin-1, CAM 12/8, Epithelial cadherin, E-cadherin, Uvomorulin, CDH1

Background: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

ID gène: 999

UniProt: P12830