CD20 anticorps (CF®488A)
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- Antigène Voir toutes CD20 (MS4A1) Anticorps
- CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD20 est conjugé à/à la CF®488A
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Test (T)
- Specificité
- Recognizes a protein of 33-37 kDa, identified as CD20 (Workshop V, Code CD20.12). B9E9 recognizes extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, B cell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.
- Purification
- Purified by Protein A/G
- Immunogène
- Lymphoblastoid cell line Daudi
- Clone
- B9E9
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product MS4A1 Anticorps primaire
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- Indications d'application
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Positive Control: Daudi, Raji and U266 and human lymphocytes.
Known Application: Flow Cytometry: 5 µL per test per one million cells (or 5 µL per 100 µL of whole blood)Immunofluorescence (1:50-1:100)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 100 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA and 0.05 % azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
- Stockage commentaire
- Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Date de péremption
- 24 months
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- Antigène
- CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
- Autre désignation
- MS4A1 (MS4A1 Produits)
- Synonymes
- anticorps B1, anticorps Bp35, anticorps CD20, anticorps CVID5, anticorps LEU-16, anticorps MS4A2, anticorps S7, anticorps AA960661, anticorps Cd20, anticorps Ly-44, anticorps Ms4a2, anticorps MS4A1, anticorps bp35, anticorps cd20, anticorps ms4a2, anticorps leu-16, anticorps ms4a4c, anticorps cd20-like, anticorps membrane spanning 4-domains A1, anticorps membrane-spanning 4-domains, subfamily A, member 1, anticorps B-lymphocyte antigen CD20, anticorps MS4A1, anticorps Ms4a1, anticorps ms4a1, anticorps LOC101694281
- Poids moléculaire
- 33-37kDa
- ID gène
- 931
- UniProt
- P11836
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