ABO anticorps

N° du produit ABIN6936339

L’anticorps Souris Monoclonal anti-ABO a été validé pour AGG, H et IHC (f). Il convient pour détecter ABO dans des échantillons de Humain.

Aperçu rapide pour ABO anticorps (ABIN6936339)

Antigène: ABO (Histo-blood group A transferase (ABO))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ABO est non-conjugé

Application: Agglutination (AGG), Haemagglutination (H), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: HEB-20

Détail du produit anti-ABO anticorps

Specificité: The antibody HEB-20 reacts with human blood group B. The specificity of the antibody HEB-20 was confirmed by comparison of specificity and reactivity to standard reagent using 5.000 samples of blood. The MAb HEB-20 shows specific staining of erythrocytes and vascular epithelium of blood group B controls and no staining in group A controls. This MAb is applicable for tissue staining in tumor patients with blood groups B and AB. Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Mixture of erythrocytes of group B and glyco protein fraction isolated from saliva of secretors with blood group B

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Agglutination, Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: KG1 cells. Human colorectal carcinoma tissues.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur ABO

Antigène: ABO (Histo-blood group A transferase (ABO))

Autre désignation: ABO

Sujet: A transferase, ABO, B transferase, CD173, Fucosylglycoprotein 3-alpha-galactosyltransferase, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-galactosyltransferase, Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase, Histo-blood group A transferase, Histo-blood group B transferase, NAGAT,Blood Group Antigen B (CD173)
Cellular localisation: Cell Surface

Poids moléculaire: Multiple

ID gène: 28, 654423

UniProt: P16442