Calpastatin anticorps

N° du produit ABIN6936579

L’anticorps Souris Monoclonal anti-Calpastatin a été validé pour ELISA, Coat et IHC (f). Il convient pour détecter Calpastatin dans des échantillons de Humain.

Aperçu rapide pour Calpastatin anticorps (ABIN6936579)

Antigène: Calpastatin (CAST)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Calpastatin est non-conjugé

Application: ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: CAST-1550

Détail du produit anti-Calpastatin anticorps

Specificité: Calpastatin is an intracellular calcium-dependent protease that cleaves cytoskeletal and sub-membranous proteins. Calpains are non-lysosomal, calcium-activated intracellular cysteine proteases. Calpains mediate specific Ca2+- dependent processes including cell fusion, mitosis and meiosis. Calpains are heterodimers of a small regulatory subunit and one of three large catalytic subunits, designated Calpastatin, Calpain 2 and Calpain p94. Calpastatin regulates Calpain by inhibiting both the proteolytic activity of Calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing. Calpastatin co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain. Calpain influences the process of spermatogenesis and the events preceding fertilization, such as the acrosome reaction.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human CAST protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Esophagus or Small Intestine.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Calpastatin

Antigène: Calpastatin (CAST)

Autre désignation: CAST

Sujet: BS 17, Calpain inhibitor, Calpastatin, Cast, Heart type calpastatin, Sperm BS 17 component,Calpastatin
Cellular localisation: Cytoplasm and Cell Surface

Poids moléculaire: 126kDa

ID gène: 831, 436186

UniProt: P20810