CD81 anticorps

N° du produit ABIN6936836

Cet anticorps Souris Monoclonal détecte spécifiquement CD81 dans FACS, WB, IF, Func et IHC (f). Il présente une réactivité envers Humain.

Aperçu rapide pour CD81 anticorps (ABIN6936836)

Antigène: CD81

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD81 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 1-3-3-22

Détail du produit anti-CD81 anticorps

Specificité: Recognizes a protein of 26 kDa, identified as CD81 (Workshop VI, Code CD81.1)). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.

Réactivité croisée (Details): Human. Mouse. Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human CD81 protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Functional Studies (Order Ab without Azide),Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL for 2 hours at RT),Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7, Ramos, Daudi or Jurkat cells. Human lymphocytes. Human tonsil or liver.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur CD81

Antigène: CD81

Autre désignation: CD81

Sujet: CD81, Target of the antiproliferative antibody 1 (TAPA-1), Tetraspanin-28, Tspan-28,CD81 / TAPA-1
Cellular localisation: Cell surface

Poids moléculaire: 26kDa

ID gène: 975, 54457

UniProt: P60033

Pathways: Inositol Metabolic Process, Hepatitis C