Recombinant CD81 anticorps

N° du produit ABIN6936837

L’anticorps Lapin Monoclonal anti-CD81 a été validé pour FACS et IHC (f). Il convient pour détecter CD81 dans des échantillons de Humain.

Aperçu rapide pour Recombinant CD81 anticorps (ABIN6936837)

Antigène: CD81

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp CD81 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: C81-2885R

Détail du produit anti-CD81 anticorps

Specificité: Recognizes a protein of 26 kDa, identified as CD81 (Workshop VI, Code CD81.1)). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.

Réactivité croisée (Details): Human, Mouse and Rat. Others not known.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: B-Cell line derived from a Burkitt lymphoma

Isotype: IgG

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (0.5-1 μg/million cells), Immunohistology (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7, Ramos, Daudi, Raji, U266, HUT-78, HUT-102, Jurkat, HL-60, KG1a, A293, A431, MG63, and human lymphocytes. Tonsil or Liver.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur CD81

Antigène: CD81

Autre désignation: CD81 / TAPA-1

Sujet: CD81, Target of the antiproliferative antibody 1 (TAPA-1), Tetraspanin-28, Tspan-28,CD81 / TAPA-1
Cellular localisation: Cell surface

Poids moléculaire: 26kDa

ID gène: 975, 54457

UniProt: P60033

Pathways: Inositol Metabolic Process, Hepatitis C