CD99 anticorps

N° du produit ABIN6936869

Cet anticorps Lapin Polyclonal détecte spécifiquement CD99 dans IHC (f). Il présente une réactivité envers Humain.

Aperçu rapide pour CD99 anticorps (ABIN6936869)

Antigène: CD99

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CD99 est non-conjugé

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-CD99 anticorps

Specificité: Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32).Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis.CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing's sarcoma cells and primitive peripheral neuroectodermal tumors.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant human MIC2 protein

Isotype: IgG

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (5-10ug/ml for 30 min at Room Temp)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.

Positive_Control: MOLT-4 cells. Pancreas or Ewing's sarcoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur CD99

Antigène: CD99

Autre désignation: CD99

Sujet: 12E7; E2 antigen; MIC 2X; MIC 2Y; MIC2; Protein MIC2; Surface antigen MIC2; T-cell surface glycoprotein E2,CD99 / MIC2 (Ewing's Sarcoma Marker)
Cellular localisation: Cell surface

Poids moléculaire: 27-32kDa

ID gène: 4267, 653349

UniProt: P14209