CGA (HCG-Alpha) & CGB (HCG-Beta) anticorps

N° du produit ABIN6936962

Cet anticorps Souris Monoclonal détecte spécifiquement CGA (HCG-Alpha) & CGB (HCG-Beta) dans IHC (f). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour CGA (HCG-Alpha) & CGB (HCG-Beta) anticorps (ABIN6936962)

Antigène: CGA (HCG-Alpha) & CGB (HCG-Beta)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: HCGab-52

Détail du produit

Specificité: This MAb is a very special because it reacts ONLY with the intact-HCG and not with either free alpha- or free beta-chain of HCG. HCG is a glycoprotein and is composed of two non-identical, non-covalently linked polypeptide chains designated as the subunit is identical to that of thyroid stimulating hormone (TSH) follicle stimulating hormone (FSH), and luteinizing hormone (LH). HCG is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG MAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90 % and 60 % of cases respectively. 20 % of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Purified hCG protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: JAR or TT cells. Placenta.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détail du antigène

Antigène: CGA (HCG-Alpha) & CGB (HCG-Beta)

Sujet: CG-alpha, CGA, Chorionic Gonadotrophin Alpha, Follicle Stimulating Hormone Alpha, Follitropin Alpha, FSH-alpha, FSHA, GPH Alpha, GPHA1, LHA, LH-alpha, Luteinizing Hormone Alpha, Lutropin Alpha, Thyroid Stimulating Hormone Alpha, Thyrotropin Alpha, TSHA, CG-beta, CGB3, CGB5, CGB7, CGB8, Choriogonadotropin Subunit beta, hCGB,HCG Holo (Pregnancy & Choriocarcinoma Marker)
Cellular localisation: Cytoplasmic, Secreted

Poids moléculaire: ~35kDa (intact hCG)

ID gène: 1081, 1082, 172944

UniProt: P01215, P01233