CNN1 anticorps

N° du produit ABIN6937005

L’anticorps anti-CNN1 Monoclonal Souris est utilisé pour la détection de CNN1 dans des échantillons de Humain et Rat. Il a été validé pour IHC, FACS, IF et WB.

Aperçu rapide pour CNN1 anticorps (ABIN6937005)

Antigène: CNN1 (Calponin 1 (CNN1))

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CNN1 est non-conjugé

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)

Clone: CALP

Détail du produit anti-CNN1 anticorps

Fonction: Calponin-1 (Smooth Muscle Marker) Antibody

Specificité: Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34 kDa form and a 29 kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34 kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.

Réactivité croisée (Details): Human. Rat.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Crude human uterus extract

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1 mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: K562 cells, Myoepithelial cells in breast ducts.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CNN1

Antigène: CNN1 (Calponin 1 (CNN1))

Autre désignation: CNN1

Sujet:

Calponin 1 basic smooth muscle, Calponin H1 smooth muscle, Calponin-1, CNN1, Cnn1, Sm Calp, SMCC

K562 cells or Colon, Myoepithelial cells in breast ducts.

Poids moléculaire: 34kDa

ID gène: 1264, 465929

UniProt: P51911