Keratin 5,6,18 anticorps

N° du produit ABIN6937075

Cet anticorps anti-Keratin 5,6,18 Monoclonal Souris (Clone SPM267) (ABIN6937075) détecte spécifiquement Keratin 5,6,18 dans IHC, WB, FACS et IF. L’anticorps est réactif avec des échantillons de Humain, Rat et Boeuf (Vache).

Aperçu rapide pour Keratin 5,6,18 anticorps (ABIN6937075)

Antigène: Keratin 5,6,18

Reactivité: Humain, Rat, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: SPM267

Détail du produit

Fonction: Cytokeratin 5/6/18 Antibody

Specificité: It recognizes polypeptides of 58 kDa, 56 kDa, and 45 kDa, identified as cytokeratin 5, 6, and 18 respectively. It shows no reaction with keratin 1, 8, or 19. This MAb shows a broad pattern of reactivity with epithelial tissues, from simple glandular epithelia to stratified squamous epithelia. Epithelial cells are labeled whether they are ectodermal, mesodermal, or endodermal in origin. This MAb is useful in detecting cells of epithelial origin in mixed tumors, in effusions, in bone marrow samples, or in tissue culture cells.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Detergent-insoluble fraction of psoriatic human epidermis

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Positive Control: HeLa, HepG2 or PC12 cells. Tonsil or Skin.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détail du antigène

Antigène: Keratin 5,6,18

Autre désignation: Cytokeratin 5/6/18

Sujet:

CK5, Keratin-5, KRT5, Cytoskeletal 6, KRT6, CK18, CYK18, K18, Keratin-18, KRT18

HeLa, HepG2 or PC12 cells. Tonsil or Skin.

Poids moléculaire: 45.58kDa