Recombinant Cytokeratin (KRT) anticorps

N° du produit ABIN6937080

L’anticorps anti-Cytokeratin Monoclonal Lapin est utilisé pour la détection de Cytokeratin dans des échantillons de Humain, Rat, Porc, Souris, Singe, Cobaye, Chévre, Grenouille et Boeuf (Vache). Il a été validé pour WB, FACS, IHC et IF.

Aperçu rapide pour Recombinant Cytokeratin (KRT) anticorps (ABIN6937080)

Antigène: Cytokeratin (KRT)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Rat, Porc, Souris, Singe, Cobaye, Chévre, Grenouille, Boeuf (Vache)

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: KRT-1877R

Détail du produit

Fonction: Recombinant Cytokeratin, Multi (Epithelial Marker) Antibody

Specificité: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13) and 45 kDa (CK18). This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Keratin-enriched preparation from cultured human epithelial cells

Isotype: IgG, kappa

Information d'application

Indications d'application:

Positive Control: A431 cells, Skin, Colon carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détail du antigène

Antigène: Cytokeratin (KRT)

Autre désignation: Cytokeratin

Sujet: A431 cells, Colon carcinoma., Skin

ID gène: 3851, 3852, 3853

UniProt: P02538, P04259, P13647, P19013, P48668