Recombinant Cytokeratin, Multi (Epithelial Marker) anticorps

N° du produit ABIN6937080

L’anticorps Lapin Monoclonal anti- a été validé pour IHC, StM, FACS, IF et WB. Il convient pour détecter dans des échantillons de Boeuf (Vache), Grenouille, Chévre, Cobaye, Humain, Singe, Souris, Porc et Rat.

Aperçu rapide pour Recombinant Cytokeratin, Multi (Epithelial Marker) anticorps (ABIN6937080)

Antigène: Cytokeratin, Multi (Epithelial Marker)

Type d'anticorp: Recombinant Antibody

Reactivité: Boeuf (Vache), Grenouille, Chévre, Cobaye, Humain, Singe, Souris, Porc, Rat

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunohistochemistry (IHC), Staining Methods (StM), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)

Clone: KRT-1877R

Détail du produit

Specificité: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13) and 45 kDa (CK18). This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Purification: Purified by Protein A/G

Immunogène: Keratin-enriched preparation from cultured human epithelial cells

Isotype: IgG

Information d'application

Indications d'application:

Positive Control: A431 cells, Skin, Colon carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Buffer: 10 mM PBS without BSA and without Azide.

Agent conservateur: Azide free

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Date de péremption: 24 months

Détail du antigène

Antigène: Cytokeratin, Multi (Epithelial Marker)

ID gène: 3851, 3852, 3853

UniProt: P02538, P04259, P13647, P19013, P48668