DMC1 anticorps

N° du produit ABIN6937090

Détail du produit anti-DMC1 anticorps

Antigène: DMC1 (DNA Meiotic Recombinase 1 (DMC1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp DMC1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: DNA repair proteins are necessary for the maintenance of chromosome integrity and are involved in the elimination of premutagenic lesions from DNA. The DNA repair proteins Rad51 and Rad52 are key components of the doublestrand-break repair (DSBR) pathway. Rad51 is essential for mitotic and meiotic recombination, and its mutation in yeast and mammalian cells results in chromosome loss. Overexpression of Rad52 confers resistance to ionizing radiation and induces homologous intrachromosomal recombination. Rad52 is thought to be involved in an early stage of Rad51-mediated recombination. Additional proteins involved in the pathway include Nibrin and Dmc1. Nibrin, which complexes with Mre11 and Rad50, is absent in Nijemegen breakage syndrome (NBS) patients. Dmc1 is specifically involved in meiotic recombination. An alternative spliced form of Dmc1, designated Dmc1-D, is deleted for a region between the two motifs involved in nucleotide binding. The alternatively spliced Dmc1-D transcript is detected in both male and female germ cells, indicating that the encoded protein may have a role in mammalian genetic recombination in meiosis.

Réactivité croisée (Details): Human. Mouse. Rat. Cow.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human DMC1 protein.

Clone: 2H12-4

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Known_Application: Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Cervix or prostate.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur DMC1

Antigène: DMC1 (DNA Meiotic Recombinase 1 (DMC1))

Autre désignation: DMC1 (DMC1 Produits)

Synonymes: anticorps DMC1, anticorps zgc:136628, anticorps Dmc1h, anticorps Lim15, anticorps Mei11, anticorps sgdp, anticorps DCM1, anticorps DMC1H, anticorps LIM15, anticorps dJ199H16.1, anticorps DNA meiotic recombinase 1, anticorps DMC1, anticorps dmc1, anticorps Dmc1

Sujet: Disrupted meiotic cDNA 1 homolog, dJ199H16.1, DMC1 dosage suppressor of mck1 homolog, DMC1H, DNA meiotic recombinase 1, HsLim15, LIM15, Meiotic recombination protein DMC1/LIM15 homolog, MGC150472, MGC150473,DMC1
Cellular localisation: Nucleus. Chromosome.

Poids moléculaire: 37kDa

ID gène: 11144, 339396

UniProt: Q14565