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dsDNA anticorps

dsDNA Reactivité: Humain IF, FACS, IHC, StM Hôte: Souris Monoclonal 121-3 unconjugated
N° du produit ABIN6937097
  • Antigène Tous les produits dsDNA
    dsDNA (Double-Stranded DNA (dsDNA))
    Reactivité
    • 13
    • 2
    • 1
    Humain
    Hôte
    • 14
    • 2
    Souris
    Clonalité
    • 14
    • 2
    Monoclonal
    Conjugué
    • 9
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp dsDNA est non-conjugé
    Application
    • 11
    • 10
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Staining Methods (StM)
    Specificité
    This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This MAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells.Deoxyribonucleic acid (DNA) is a long polymer of nucleotides that is held together by a backbone made of sugars and phosphate groups. It holds the genetic instructions for the development and function of living things. DNA is crucial for living organisms, and all known cellular life and some viruses contain DNA. In eukaryotes, DNA exists in the cell nucleus, while in prokaryotes, DNA is located in the cytoplasm. In living organisms, DNA does not usually exist as a single molecule, but instead as a tightly associated pair of molecules in the shape of a right-handed double helix. Hydrogen bonds as well as forces generated by the hydrophobic effect and pi stacking hold the two DNA strands together. During replication and transcription, portions of the helix unwind and become single stranded. Protective proteins surround these single-stranded DNA. Double stranded (ds) DNA markers are useful tools in biology research and aid in the study of DNA behavior and characteristics.
    Immunogène
    Nuclei of Burkitt's cells
    Clone
    121-3
    Isotype
    IgG3 kappa
  • Indications d'application

    Positive Control: Raji, Jurkat or HeLa cells. Tonsil or Colon.

    Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Buffer
    10 mM PBS without BSA and without Azide.
    Agent conservateur
    Azide free
    Stock
    4 °C,-80 °C
    Stockage commentaire
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.
    Date de péremption
    24 months
  • Antigène
    dsDNA (Double-Stranded DNA (dsDNA))
    Autre désignation
    Double Stranded DNA (dsDNA) (Nuclear Marker) (dsDNA Produits)
    Classe de substances
    Chemical
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