Desmoglein 1 anticorps

N° du produit ABIN6937109

Cet anticorps Souris Monoclonal détecte spécifiquement Desmoglein 1 dans IHC (f). Il présente une réactivité envers Boeuf (Vache).

Aperçu rapide pour Desmoglein 1 anticorps (ABIN6937109)

Antigène: Desmoglein 1 (DSG1)

Reactivité: Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Desmoglein 1 est non-conjugé

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 32-2B

Détail du produit anti-Desmoglein 1 anticorps

Specificité: Recognizes a protein of ~150 kDa, identified as Desmoglein-1 (DSG1). Desmoglein-1 is a member of the desmosomal cadherin family.Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms.Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal layers. Intact and functionally active desmoglein 1 is essential to epidermal integrity.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Bovine desmoglein (desmosomal glyco protein I) from nasal epidermis.

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Bladder, skin or melanoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Desmoglein 1

Antigène: Desmoglein 1 (DSG1)

Autre désignation: DSG1

Sujet: Cadherin family member 4, CDHF4, Desmoglein-1, Desmosomal glycoprotein 1, DG1, DSG1, EPKHE, EPKHIA, Pemphigus foliaceus antigen, PPKS1, SPPK1,Desmoglein-1 (DSG1)
Cellular localisation: Cell surface

Poids moléculaire: 150kDa (precursor), 160kDa (mature)

ID gène: 1828, 2633

UniProt: Q02413

Pathways: Cell-Cell Junction Organization