Desmoglein 3 anticorps

N° du produit ABIN6937113

L’anticorps anti-Desmoglein 3 Monoclonal Souris est utilisé pour la détection de Desmoglein 3 dans des échantillons de Humain. Il a été validé pour WB, IHC, IF et FACS.

Aperçu rapide pour Desmoglein 3 anticorps (ABIN6937113)

Antigène: Desmoglein 3 (DSG3)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Desmoglein 3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 5G11

Détail du produit anti-Desmoglein 3 anticorps

Fonction: Desmoglein-3 (Squamous Cell Marker) Antibody

Specificité: Recognizes a protein of 130 kDa, identified as Desmoglein-3 (DSG3). This MAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, three desmoglein subfamily members are identified and all are members of the cadherin cell adhesion molecule superfamily.

Réactivité croisée (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant human DSG3 extracellular protein fragment

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: A431 cells. Lung squamous cell carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Desmoglein 3

Antigène: Desmoglein 3 (DSG3)

Autre désignation: DSG3

Sujet:

130 kDa pemphigus vulgaris antigen (PVA), Balding (Bal), Cadherin family member 6 (CDHF6), Desmoglein-3 (DSG3)

A431 cells. Lung squamous cell carcinoma.

Poids moléculaire: 130kDa

ID gène: 1830, 1925

UniProt: P32926