Filaggrin anticorps (AA 198-288)

N° du produit ABIN6937230

Détail du produit anti-Filaggrin anticorps

Antigène: Filaggrin (FLG)

Épitope: AA 198-288

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Filaggrin est non-conjugé

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules.Active filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant human Filaggrin protein fragment (aa 198-288) (exact sequence is proprietary)

Clone: FLG-1561

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: U-251-MG cells. Skin.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Filaggrin

Antigène: Filaggrin (FLG)

Autre désignation: FLG (FLG Produits)

Synonymes: anticorps AW107830, anticorps ft, anticorps FLG, anticorps ATOD2, anticorps Pflg, anticorps Flg, anticorps filaggrin, anticorps Flg, anticorps FLG, anticorps LOC100351762, anticorps LOC100728006

Sujet: ATOD2, Epidermal Filaggrin, Filaggrin, Filaggrin precursor, Fillagrin, FLG, Profilaggrin,Filaggrin (Keratinocyte Differentiation Marker)
Cellular localisation: Cytoplasmic

Poids moléculaire: 26-45kDa (Processed), 350kDa (Profilaggrin)

ID gène: 2312, 654510

UniProt: P20930

Pathways: Sensory Perception of Sound, Stem Cell Maintenance