FOXA1 anticorps (AA 372-472)

N° du produit ABIN6937255

Cet anticorps anti-FOXA1 Monoclonal Souris (Clone FOXA1-1514) (ABIN6937255) détecte spécifiquement FOXA1 dans WB, IF et FACS. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour FOXA1 anticorps (AA 372-472) (ABIN6937255)

Antigène: FOXA1 (Forkhead Box A1 (FOXA1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FOXA1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: FOXA1-1514

Détail du produit anti-FOXA1 anticorps

Épitope: AA 372-472

Fonction: FOXA1 / HNF3A Antibody

Specificité: The transcription factor Forkhead-box A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors. HNF-1 (°™ and æ), HNF-3 (°™, æ and Á), HNF-4 (°™ and Á), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. FOXA1 expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.

Aucune reactivité croisée: Rat (Rattus)

Réactivité croisée (Details): Human. Does not react with Rat. Others not known.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant human FOXA1 protein fragment (aa372-472) (exact sequence is proprietary)

Isotype: IgG2b, kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), ,Western Blotting (0.5-1 μg/mL), ,Immunohistology (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HepG-2 or MCF-7 cells. Liver, Pancreas, Bladder, Prostate, Colon and Lung and Breast.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur FOXA1

Antigène: FOXA1 (Forkhead Box A1 (FOXA1))

Autre désignation: FOXA1

Sujet:

Forkhead box protein A1 (FOXA1), Hepatocyte nuclear factor 3-alpha (HNF-3-alpha or HNF-3A or HNF3A), Transcription factor 3A (TCF-3A or TCF3A)

Bladder, Colon and Lung and Breast., HepG-2 or MCF-7 cells. Liver, Pancreas, prostate

Poids moléculaire: 49kDa

ID gène: 3169, 163484

UniProt: P55317

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis