FTH1 anticorps (AA 58-180)

N° du produit ABIN6937271

Cet anticorps Souris Monoclonal détecte spécifiquement FTH1 dans WB et IHC (f). Il présente une réactivité envers Humain.

Aperçu rapide pour FTH1 anticorps (AA 58-180) (ABIN6937271)

Antigène: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FTH1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: FTH-2081

Détail du produit anti-FTH1 anticorps

Épitope: AA 58-180

Specificité: Mammalian ferritins consist of 24 subunits made up of 2 types of poly-peptide chains, ferritin heavy chain and ferritin light chain, which each have unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidationof FeI(II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe(III). The most prominent role of mammalian ferritins is to provide iron-buffering capacity to cells. In addition to iron buffering, heavy chain ferritin is also involved in the regulation of thymidine biosynthesis via increased expression of cytoplasmic serine hydroxymethyltransferase, which is a limiting factor in thymidylate synthesis in MCF-7 cells. Light chain ferritin is involved in cataracts by at least two mechanisms: hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of aging-related cataracts.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant human FTH1 protein fragment (around aa 58-180) (exact sequence is proprietary)

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HepG2, HeLa, HL-60 or 293T cells. Pancreas, Liver, Cerebellum or Testis.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur FTH1

Antigène: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Autre désignation: FTH1

Sujet: Apoferritin, Cell proliferation-inducing gene 15 protein, Ferritin H subunit, Ferritin heavy chain, FHC, FRIH, FTH1, FTHL6, Iron overload autosomal dominant, PIG15, Placenta immunoregulatory factor, Proliferation inducing gene 15 protein (PLIF),Ferritin, Heavy Chain (FTH) (Microglia Marker)
Cellular localisation: Cytoplasmic

Poids moléculaire: 21kDa

ID gène: 2495, 524910, 645560

UniProt: P02794

Pathways: Transition Metal Ion Homeostasis