GFAP anticorps

N° du produit ABIN6937306

Cet anticorps anti-GFAP est un anticorps Souris Monoclonal détectant GFAP dans WB, IHC, IF, FACS et IHC (f). Adapté pour Porc.

Aperçu rapide pour GFAP anticorps (ABIN6937306)

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivité: Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GFAP est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: GA-5

Détail du produit anti-GFAP anticorps

Specificité: This MAb recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Réactivité croisée (Details): Human. Mouse. Rat. Cow. Pig. Rabbit. Chicken.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: GFAP isolated from pig spinal cord

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: T98G cells (FACS). Brain lysate (WB). Brain or Astrocytoma (IHC).

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur GFAP

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Autre désignation: GFAP

Sujet: Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP),GFAP (Astrocyte & Neural Stem Cell Marker)
Cellular localisation: Cytoplasmic

Poids moléculaire: ~50kDa

ID gène: 2670, 514227

UniProt: P14136