GFAP anticorps (AA 101-200)
Aperçu rapide pour GFAP anticorps (AA 101-200) (ABIN6937311)
Antigène
Voir toutes GFAP AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Épitope
- AA 101-200
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Fonction
- GFAP (Astrocyte & Neural Stem Cell Marker) Antibody
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Specificité
- This MAb recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
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Réactivité croisée (Details)
- Human.
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Purification
- 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.
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Immunogène
- Recombinant human GFAP protein fragment (around aa 101-200) (exact sequence is proprietary)
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Isotype
- IgG1, kappa
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Indications d'application
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Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: T98G cells (FACS). Brain lysate (WB). Brain or Astrocytoma (IHC).
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Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- Prepared in 10 mM PBS
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Agent conservateur
- Azide free
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Stock
- -20 °C,-80 °C
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Stockage commentaire
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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Date de péremption
- 24 months
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- GFAP (Glial Fibrillary Acidic Protein (GFAP))
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Autre désignation
- GFAP
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Sujet
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Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)
T98G cells (FACS). Human brain lysate (WB). Brain or Astrocytoma (IHC).
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Poids moléculaire
- ~50kDa
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ID gène
- 2670, 514227
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UniProt
- P14136
Antigène
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