IGHA1 & IGHA2 anticorps

N° du produit ABIN6937489

Cet anticorps anti-IGHA1 & IGHA2 Monoclonal Souris (Clone HISA43) (ABIN6937489) détecte spécifiquement IGHA1 & IGHA2 dans FACS, IF et IHC (f). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour IGHA1 & IGHA2 anticorps (ABIN6937489)

Antigène: IGHA1 & IGHA2

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: HISA43

Détail du produit

Specificité: This MAb is specific to heavy chain of IgA and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with both IgA1 and IgA2 subclasses of Alpha heavy chain. It reacts with the third constant domain (CH3) of the alpha chain of IgA molecules. Immunoglobulins are four-chain, Y-shaped, monomeric structures comprised of two identical heavy chains and two identical light chains held together through inter-chain disulfide bonds. The chains form two domains, the Fab (antigen binding) fragment and the Fc (constant) fragment. Immunoglobulin A (IgA) is the main protein of the mucosal immune system. It is generated by B-cells in gut-associated lymphoid tissues. Daily production of IgA exceeds that of any of the other immunoglobulins.IgA exists mainly in dimers but can also exist as polymers or as monomers. Dimers and polymers contain a joining (J) chain that can be bound by the polymeric immunoglobulin receptor (pIgR) for transportation of the molecule to mucosal surfaces. The most common feature of plasmacytomas, and certain non-Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Purified human IgA

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL 30 min RT), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL 30 min RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Daudi, 293T, Raji or hPBL cells. Tonsil or Spleen.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détail du antigène

Antigène: IGHA1 & IGHA2

Sujet: A2m Marker, Ig alpha 1 Chain C Region, Ig alpha 2 Chain C Region, IGHA1, IGHA2, Immunoglobulin Am1, Immunoglobulin Am2, Immunoglobulin Heavy Constant Alpha 1, Immunoglobulin Heavy Constant Alpha 2,IgA (Immunoglobulin Alpha Heavy Chain) (B-Cell Marker)
Cellular localisation: Cytoplasm, Cell Surface and Secreted

Poids moléculaire: 50-75kDa

ID gène: 3493, 3494, 699841

UniProt: P01876, P01877