IGHM anticorps

N° du produit ABIN6937512

L’anticorps anti-IGHM Monoclonal Souris est utilisé pour la détection de IGHM dans des échantillons de Humain. Il a été validé pour IHC, FACS et IF.

Aperçu rapide pour IGHM anticorps (ABIN6937512)

Antigène: IGHM (Immunoglobulin Heavy Constant mu (IGHM))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IGHM est non-conjugé

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: ICO-30

Détail du produit anti-IGHM anticorps

Fonction: IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Antibody

Specificité: Recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. IgM antibody is prominent in early immune responses to most antigens. Aberrant levels are associated with immune deficiency states, hereditary deficiencies, myeloma, Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Réactivité croisée (Details): Human.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Heavy chain of human IgM

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: 293T, Raji or hPBL cells. Tonsil or Spleen.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur IGHM

Antigène: IGHM (Immunoglobulin Heavy Constant mu (IGHM))

Autre désignation: IGHM

Sujet:

AGM1, IGHM, Constant Region of Heavy Chain of IgM, Ig Mu Chain C Region

293T, Raji or hPBL cells. Tonsil or Spleen.

Poids moléculaire: 50-75kDa

ID gène: 3507, 510635

UniProt: P01871