KRT anticorps

N° du produit ABIN6937637

Cet anticorps Souris Monoclonal détecte spécifiquement KRT dans FACS, WB, IF et IHC (f). Il présente une réactivité envers Humain.

Aperçu rapide pour KRT anticorps (ABIN6937637)

Antigène: KRT (KRT126P) (Keratin 126 Pseudogene (KRT126P))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KRT est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: Cocktail PAN-CK

Détail du produit anti-KRT anticorps

Specificité: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, with 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 55 kDa (CK7), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 46 kDa (CK17), 45 kDa (CK18) and 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRT-PAN is a broad-spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It is useful in characterizing the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and shows high sensitivity in the recognition of epithelial cells and carcinomas.

Réactivité croisée (Details): Human, Monkey, Cow, Dog, Rabbit, Mouse, Rat, Cat and Chicken.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Human epidermal keratins

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (0.5-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-2 μg/mL for 2 hours at RT),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, MCF-7, Skin, Adeno- or Squamous carcinomas

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur KRT

Antigène: KRT (KRT126P) (Keratin 126 Pseudogene (KRT126P))

Autre désignation: KRT

Sujet: K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39,Cytokeratin, pan (Epithelial Marker)
Cellular localisation: Cytoplasmic

Poids moléculaire: 40-67kDa (Multiple)