Keratin 10 anticorps
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- Antigène Voir toutes Keratin 10 (KRT10) Anticorps
- Keratin 10 (KRT10)
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Keratin 10 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificité
- This MAb recognizes a protein of 56.5 kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation, it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
- Réactivité croisée (Details)
- Human. Mouse.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogène
- Skin extract of a human Psoriasis patient
- Clone
- LH2
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product KRT10 Anticorps primaire
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- Indications d'application
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Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: A431, HeLa, MCF7 cells. Esophagus or tonsil.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Agent conservateur
- Azide free
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Date de péremption
- 24 months
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- Antigène
- Keratin 10 (KRT10)
- Autre désignation
- KRT10 (KRT10 Produits)
- Synonymes
- anticorps BCIE, anticorps BIE, anticorps CK10, anticorps EHK, anticorps K10, anticorps KPP, anticorps D130054E02Rik, anticorps K1C1, anticorps Krt-1.10, anticorps Krt1-10, anticorps Ka10, anticorps Ker10, anticorps KRT10, anticorps KRT25, anticorps KRT27, anticorps KRT28, anticorps keratin 10, anticorps keratin 10, type I S homeolog, anticorps keratin, type I cytoskeletal 25, anticorps KRT10, anticorps Krt10, anticorps krt10.S, anticorps LOC101085587
- Sujet
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BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10,Cytokeratin 10 (KRT10) (Suprabasal Epithelial Marker)
Cellular localisation: Cytoplasmic - Poids moléculaire
- 56.5kDa
- ID gène
- 3858, 99936
- UniProt
- P13645
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