KRT14 anticorps (AA 351-472)

N° du produit ABIN6937657

Cet anticorps anti-KRT14 est un anticorps Souris Monoclonal détectant KRT14 dans FACS et IHC (f). Adapté pour Humain.

Aperçu rapide pour KRT14 anticorps (AA 351-472) (ABIN6937657)

Antigène: KRT14 (Keratin 14 (KRT14))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KRT14 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: KRT14-2375

Détail du produit anti-KRT14 anticorps

Épitope: AA 351-472

Specificité: Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.

Réactivité croisée (Details): Human. Mouse. Rat. Pig.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant human KRT14 fragment (around aa351-472) (exact sequence is proprietary)

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: A431 or HeLa cells. Skin or Squamous Cell Carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur KRT14

Antigène: KRT14 (Keratin 14 (KRT14))

Autre désignation: KRT14

Sujet: CK-14, Dowling Meara, ebs3, ebs4, Epidermolysis Bullosa Simplex, k14, Keratin 14, Keratin Type I Cytoskeletal 14, Koebner, krt14, NFJ,Cytokeratin 14 (KRT14) (Squamous Cell Marker)
Cellular localisation: Cytoplasmic

Poids moléculaire: 50kDa

ID gène: 3861, 6545380

UniProt: P02533