Recombinant Cytokeratin 19 anticorps

N° du produit ABIN6937691

Cet anticorps Souris Monoclonal détecte spécifiquement Cytokeratin 19 dans WB, IF, FACS et IHC (f). Il présente une réactivité envers Humain.

Aperçu rapide pour Recombinant Cytokeratin 19 anticorps (ABIN6937691)

Antigène: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytokeratin 19 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: RKRT19-799

Détail du produit anti-Cytokeratin 19 anticorps

Specificité: This MAb reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40 kDa. Its epitope maps between amino acid 312-335. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50 % -60 % of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.

Aucune reactivité croisée: Furet, Rat (Rattus)

Réactivité croisée (Details): Human. Does not react with Rat or Ferret.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant human full-length KRT19 protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7, HeLa oe Hep-G2 cells. Human breast cancer tissue.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Cytokeratin 19

Antigène: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Autre désignation: KRT19

Sujet: K19, k1cs, Keratin 19 Keratin Type i 40kD, krt19,Cytokeratin 19 (KRT19) (Pancreatic Stem Cell Marker)
Cellular localisation: Cytoplasmic

Poids moléculaire: 40kDa

ID gène: 3880, 654568

UniProt: P08727