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Cytokeratin 2 anticorps

L’anticorps Souris Monoclonal anti-Cytokeratin 2 a été validé pour WB, IF, FACS et IHC (f). Il convient pour détecter Cytokeratin 2 dans des échantillons de Humain.
N° du produit ABIN6937731

Aperçu rapide pour Cytokeratin 2 anticorps (ABIN6937731)

Antigène

Voir toutes Cytokeratin 2 (KRT76) Anticorps
Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

Reactivité

  • 33
  • 13
  • 8
  • 1
  • 1
Humain

Hôte

  • 22
  • 10
  • 1
Souris

Clonalité

  • 20
  • 13
Monoclonal

Conjugué

  • 23
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Cytokeratin 2 est non-conjugé

Application

  • 17
  • 12
  • 10
  • 6
  • 6
  • 5
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone

DE-SQ
  • Specificité

    This antibody is specific to cytokeratin 13, 14, 15 and 16. This antibody stains squamous carcinomas and adeno-squamous carcinomas but does not stain adenocarcinoma. This antibody is useful in differential staining of squamous carcinomas from adenocarcinomas and to differentiate benign and malignant tumors of prostate glands.

    Réactivité croisée (Details)

    Human.

    Purification

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    Immunogène

    Cytoskeletal fraction prepared from human gingiva by detergent/salt extraction

    Isotype

    IgG1 kappa
  • Indications d'application

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: PC12 cells. Tonsil, Skin, Prostate, Esophagus, Ectocervix or Squamous carcinoma.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    Agent conservateur

    Azide free

    Stock

    -20 °C,-80 °C

    Stockage commentaire

    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    Date de péremption

    24 months
  • Antigène

    Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

    Autre désignation

    KRT76

    Sujet

    KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9,Cytokeratin, Basic (Type II or HMW)
    Cellular localisation: Cytoplasmic

    Poids moléculaire

    50-67kDa

    ID gène

    51350, 654392

    UniProt

    Q01546
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