Recombinant Cytokeratin 2 anticorps

N° du produit ABIN6937734

L’anticorps Lapin Monoclonal anti-Cytokeratin 2 a été validé pour WB, IF, FACS et IHC (f). Il convient pour détecter Cytokeratin 2 dans des échantillons de Humain.

Aperçu rapide pour Recombinant Cytokeratin 2 anticorps (ABIN6937734)

Antigène: Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytokeratin 2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: KRTH-2147R

Détail du produit anti-Cytokeratin 2 anticorps

Specificité: This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.

Réactivité croisée (Details): Human. Mouse. Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Solubilized keratin extract from human stratum corneum

Isotype: IgG

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7, PC12 cells, Skin, Prostate carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Cytokeratin 2

Antigène: Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

Autre désignation: KRT76

Sujet: KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9,Cytokeratin, Basic (Type II or HMW)
Cellular localisation: Cytoplasmic

Poids moléculaire: 50-67kDa

ID gène: 51350, 654392

UniProt: Q01546