KRT8 anticorps

N° du produit ABIN6937744

Cet anticorps anti-KRT8 est un anticorps Souris Monoclonal détectant KRT8 dans WB, IF, FACS et IHC (f). Adapté pour Humain.

Aperçu rapide pour KRT8 anticorps (ABIN6937744)

Antigène: KRT8 (Keratin 8 (KRT8))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KRT8 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: B22-1

Détail du produit anti-KRT8 anticorps

Specificité: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular ('ring-like, perinuclear') from ductal ('peripheral-predominant') carcinoma of the breast.

Aucune reactivité croisée: Poulet, Souris, Rat (Rattus), Xenopus laevis

Réactivité croisée (Details): Human. Sheep. Rabbit. Cow. Pig. Does not react with Mouse, Rat, Chicken or xenopus laevis.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Cytoskeletal preparation from HeLa cells

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 or A431 cells. Skin, colon, lung or breast carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur KRT8

Antigène: KRT8 (Keratin 8 (KRT8))

Autre désignation: KRT8

Sujet: CARD2, CK8, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8,Cytokeratin 8 (KRT8)
Cellular localisation: Cytoplasmic

Poids moléculaire: 52.5kDa

ID gène: 3856, 533782, 708445

UniProt: P05787