Recombinant KRT8 anticorps
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- Antigène Voir toutes KRT8 Anticorps
- KRT8 (Keratin 8 (KRT8))
- Type d'anticorp
- Recombinant Antibody
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp KRT8 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt)
- Specificité
- Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular ('ring-like, perinuclear') from ductal ('peripheral-predominant') carcinoma of the breast.
- Réactivité croisée (Details)
- Human. Sheep. Rabbit. Cow. Pig.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogène
- Recombinant full-length human KRT8 protein
- Clone
- KRT8-2174R
- Isotype
- IgG
- Top Product
- Discover our top product KRT8 Anticorps primaire
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- Indications d'application
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Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: HCT116, MCF-7 or A431 cells. Skin, colon, lung or breast carcinoma.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Agent conservateur
- Azide free
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Date de péremption
- 24 months
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- Antigène
- KRT8 (Keratin 8 (KRT8))
- Autre désignation
- KRT8 (KRT8 Produits)
- Synonymes
- anticorps CARD2, anticorps CK-8, anticorps CK8, anticorps CYK8, anticorps K2C8, anticorps K8, anticorps KO, anticorps AA960620, anticorps AL022697, anticorps AU019895, anticorps Card2, anticorps EndoA, anticorps Krt-2.8, anticorps Krt2-8, anticorps CYKER, anticorps KRT2-8, anticorps KERATIN8, anticorps ck8, anticorps cyk8, anticorps k2c8, anticorps card2, anticorps krt2-5, anticorps MGC69490, anticorps KRT8, anticorps DreK8, anticorps cb186, anticorps krt2-8, anticorps sb:cb186, anticorps wu:fa20h05, anticorps wu:fa95h10, anticorps wu:fb96c06, anticorps zf-K8, anticorps zfk8, anticorps DKFZp468F2127, anticorps keratin 8, anticorps keratin 8 S homeolog, anticorps KRT8, anticorps Krt8, anticorps krt8.S, anticorps krt8
- Sujet
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CARD2, CK8, CYK8, CYKER, Cytokeratin Endo A, DreK8, K2C8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8,Cytokeratin 8 (KRT8)
Cellular localisation: Cytoplasmic - Poids moléculaire
- 52.5kDa
- ID gène
- 3856, 533782, 708445
- UniProt
- P05787
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