MAML3 anticorps

N° du produit ABIN6937803

L’anticorps Souris Monoclonal anti-MAML3 a été validé pour ISt et IHC (f). Il convient pour détecter MAML3 dans des échantillons de Humain.

Aperçu rapide pour MAML3 anticorps (ABIN6937803)

Antigène: MAML3 (Mastermind-Like 3 (MAML3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MAML3 est non-conjugé

Application: Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: MAML3-1303

Détail du produit anti-MAML3 anticorps

Specificité: MAML3 (mastermind-like protein 3) is a nuclear speckle protein that acts as a transcriptional coactivator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML3 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML3 interacts with the ankyrin repeats of the Notch proteins Notch 1, Notch 2, Notch 3 and Notch 4.This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-J a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML3 is essential for proper Notch binding, the C-terminal domain of MAML3 is essential for transcriptional activation. Due to its involvement in cell signaling and transcriptional activation, upregulation of MAML3 is thought to be involved in oncogenesis.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant human MAML3 protein fragment

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 cells. Pancreas or Placenta. Cervix or Colon Carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur MAML3

Antigène: MAML3 (Mastermind-Like 3 (MAML3))

Autre désignation: MAML3

Sujet: CAGH3, ERDA3, GDN, MAML3, Mastermind-like protein 3, TNRC3,MAML3 (Mastermind Like Transcriptional Coactivator 3)
Cellular localisation: Cytoplasmic

Poids moléculaire: 150-170kDa

ID gène: 55534, 586165

UniProt: Q96JK9