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Melanoma Marker (MART-1 + Tyrosinase + Gp100) anticorps

Reactivité: Humain IHC, ISt, StM Hôte: Souris Monoclonal DT101-BC199-T311-HMB45 unconjugated
N° du produit ABIN6937832
  • Antigène
    Melanoma Marker (MART-1 + Tyrosinase + Gp100)
    Reactivité
    Humain
    Hôte
    • 3
    Souris
    Clonalité
    • 3
    Monoclonal
    Conjugué
    • 3
    Inconjugué
    Application
    Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)
    Specificité
    This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
    Purification
    Purified by Protein A/G
    Immunogène
    Recombinant hMART-1 protein (DT101 & BC199), Extract of pigmented melanoma metastases from lymph nodes (HMB45), Recombinant tyrosinase protein (T311)
    Clone
    DT101-BC199-T311-HMB45
    Isotype
    IgG2a kappa
  • Indications d'application

    Positive Control: SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.

    Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Buffer
    10 mM PBS without BSA and without Azide.
    Agent conservateur
    Azide free
    Stock
    4 °C,-80 °C
    Stockage commentaire
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.
    Date de péremption
    24 months
  • Antigène
    Melanoma Marker (MART-1 + Tyrosinase + Gp100)
    Poids moléculaire
    20-22kDa (doublet) (MART-1): 90-100kDa (gp100), 70-80kDa (Tyrosinase)
    ID gène
    2315, 6490
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