Melanoma Marker (MART-1 + Tyrosinase + Gp100) anticorps
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- Antigène
- Melanoma Marker (MART-1 + Tyrosinase + Gp100)
- Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Inconjugué
- Application
- Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)
- Specificité
- This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
- Purification
- Purified by Protein A/G
- Immunogène
- Recombinant hMART-1 protein (DT101 & BC199), Extract of pigmented melanoma metastases from lymph nodes (HMB45), Recombinant tyrosinase protein (T311)
- Clone
- DT101-BC199-T311-HMB45
- Isotype
- IgG2a kappa
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- Indications d'application
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Positive Control: SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.
Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Buffer
- 10 mM PBS without BSA and without Azide.
- Agent conservateur
- Azide free
- Stock
- 4 °C,-80 °C
- Stockage commentaire
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.
- Date de péremption
- 24 months
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- Antigène
- Melanoma Marker (MART-1 + Tyrosinase + Gp100)
- Poids moléculaire
- 20-22kDa (doublet) (MART-1): 90-100kDa (gp100), 70-80kDa (Tyrosinase)
- ID gène
- 2315, 6490
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