Mitochondrial Marker anticorps

N° du produit ABIN6937846

Détail du produit

Antigène: Mitochondrial Marker

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunostaining (ISt), Immunohistochemistry (IHC), Staining Methods (StM)

Specificité: This MAb recognizes a 60 kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in cells from a wide variety of animals, but not insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Aucune reactivité croisée: Bactéries, Insectes

Réactivité croisée (Details): Shows broad species reactivity. Does not react with insects or bacteria.

Purification: Purified by Protein A/G

Immunogène: Mitochondrial fraction of HeLa cells

Clone: MTC719

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: HeLa or HepG2 cells. Hepatic carcinomas.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.25-0.5 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Buffer: 10 mM PBS without BSA and without Azide.

Agent conservateur: Azide free

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months.

Date de péremption: 24 months

Détail du antigène

Antigène: Mitochondrial Marker

Poids moléculaire: 60kDa