MLANA anticorps

N° du produit ABIN6937855

Cet anticorps anti-MLANA Monoclonal Souris (Clone SPM342) (ABIN6937855) détecte spécifiquement MLANA dans IHC, IF et FACS. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour MLANA anticorps (ABIN6937855)

Antigène: MLANA (Melan A (MLANA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MLANA est non-conjugé

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: SPM342

Détail du produit anti-MLANA anticorps

Fonction: MART-1 / Melan-A / MLANA (Melanoma Marker) Antibody

Specificité: This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Aucune reactivité croisée: Souris, Rat (Rattus)

Réactivité croisée (Details): Human. Horse. Does not react with Mouse or Rat.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant hMART-1 protein

Isotype: IgG2b, lambda

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur MLANA

Antigène: MLANA (Melan A (MLANA))

Autre désignation: MLANA

Sujet:

Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA

SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.

Poids moléculaire: 20-22kDa (doublet)

ID gène: 2315, 154069

UniProt: Q16655