MLANA & SILV anticorps
Aperçu rapide pour MLANA & SILV anticorps (ABIN6937867)
Antigène
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Specificité
- This antibody cocktail recognizes two melanoma-specific proteins, which include MART-1 and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. A cocktail of these two markers is sensitive for labeling formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
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Réactivité croisée (Details)
- Human.
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Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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Immunogène
- Recombinant hMART-1 protein (DT101 & BC199), Extract of pigmented melanoma metastases from lymph nodes (HMB45)
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Isotype
- IgG1 kappa
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anti-MLANA & SILV antibody
Reactivité: Humain IHC, ISt, StM Hôte: Souris Monoclonal DT101-BC199-HMB45 unconjugated
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Indications d'application
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.
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Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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Agent conservateur
- Azide free
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Stock
- -20 °C,-80 °C
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Stockage commentaire
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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Date de péremption
- 24 months
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