CD20 anticorps

N° du produit ABIN6937893

L’anticorps Souris Monoclonal anti-CD20 a été validé pour FACS, IF, Func, BP et ISt. Il convient pour détecter CD20 dans des échantillons de Humain.

Aperçu rapide pour CD20 anticorps (ABIN6937893)

Antigène: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD20 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func), Blocking Peptide (BP), Immunostaining (ISt)

Clone: B9E9

Détail du produit anti-CD20 anticorps

Specificité: Recognizes a protein of 33-37 kDa, identified as CD20 (Workshop V, Code CD20.12). B9E9 recognizes extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, B cell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Lymphoblastoid cell line Daudi

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Known_Application: Functional Studies (Order Ab without BSA & Azide), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: Daudi, Raji and U266 and human lymphocytes. In normal lymph nodes and tonsils, CD20 is predominantly detected on B cells in germinal centers.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur CD20

Antigène: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Autre désignation: MS4A1

Sujet: APY, ATOPY, B-lymphocyte antigen CD20, B-lymphocyte cell-surface antigen B1, Bp35, Fc epsilon receptor I beta chain, Fc Fragment of IgE high affinity I receptor for beta polypeptide, FCER1B, High affinity immunoglobulin epsilon receptor subunit beta, IgE Fc receptor subunit beta, IGEL, IGER, IGHER, LEU16, Leukocyte surface antigen Leu-16, Ly44, Membrane spanning 4 domains subfamily A member 2, Membrane-spanning 4-domains subfamily A member 1 (MS4A1),CD20 / MS4A1 (B-Cell Marker)
Cellular localisation: Predominantly cell surface with some cytoplasmic

Poids moléculaire: 33-37kDa

ID gène: 931, 712553

UniProt: P11836