Recombinant MTAP anticorps (AA 97-196)

N° du produit ABIN6937917

L’anticorps Souris Monoclonal anti-MTAP a été validé pour IHC, ISt et IHC (f). Il convient pour détecter MTAP dans des échantillons de Humain.

Aperçu rapide pour Recombinant MTAP anticorps (AA 97-196) (ABIN6937917)

Antigène: MTAP (Methylthioadenosine phosphorylase (MTAP))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MTAP est non-conjugé

Application: Immunohistochemistry (IHC), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: RMTAP-1813

Détail du produit anti-MTAP anticorps

Épitope: AA 97-196

Specificité: Recognizes a protein of 31kDa, which is identified as MTAP (5'-deoxy-5'-methylthioadenosine phosphorylase). It catalyzes the reversible phosphorolysis of methylthioadenosine, which is important in polyamine metabolism and for the salvage of adenine and methionine. The gene encoding MTAP is linked to the tumor suppressor gene, p16INK4A. Deficient levels of MTAP can occur in cancers primarily through co-deletion of the MTAP gene and the p16INK4A gene. Cells expressing MTAP and possessing adenine salvage pathway activity may be less susceptible to malignancy due to growth-inhibitory actions of agents (e.g. antifolates), whose mechanism of action, in part, involves this de novo purine pathway.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant human MTAP protein fragment (aa97-196) (exact sequence is proprietary)

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HepG2, A431, HeLa or MCF-7 cells. Human kidney tissues (IHC).

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur MTAP

Antigène: MTAP (Methylthioadenosine phosphorylase (MTAP))

Autre désignation: MTAP

Sujet: BDMF, DMSFH, DMSMFH, Epididymis luminal protein 249, HEL249, LGMBF, MeSAdo phosphorylase, Methylthioadenosine phosphorylase, MSAP, MTA phosphorylase, MTAPase, S-methyl-5''-thioadenosine phosphorylase,MTAP (Tumor Suppressor Marker)
Cellular localisation: Cytoplasmic

Poids moléculaire: 31kDa

ID gène: 4507, 193268

UniProt: Q13126

Pathways: Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process