MVP anticorps

N° du produit ABIN6937973

Cet anticorps Souris Monoclonal détecte spécifiquement MVP dans FACS, IF et IHC (f). Il présente une réactivité envers Humain.

Aperçu rapide pour MVP anticorps (ABIN6937973)

Antigène: MVP (Major Vault Protein (MVP))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MVP est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 1032

Détail du produit anti-MVP anticorps

Specificité: Recognizes a protein of 104 kDa-110 kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for 70 % of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: proteins precipitated from human breast cancer MCF-7 cells

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1 mM EDTA buffer, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 or HeLa cells. Breast tumors.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur MVP

Antigène: MVP (Major Vault Protein (MVP))

Autre désignation: MVP

Sujet: Lung resistance-related protein (LRP), Major Vault Protein, MVP, VAULT1,Major Vault Protein (MVP)
Cellular localisation: Cytoplasmic

Poids moléculaire: 104-110kDa

ID gène: 9961, 632177

UniProt: Q14764