MVP anticorps
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- Antigène Voir toutes MVP Anticorps
- MVP (Major Vault Protein (MVP))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp MVP est non-conjugé
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificité
- Recognizes a protein of 104 kDa-110 kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for 70 % of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.
- Réactivité croisée (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogène
- proteins precipitated from human breast cancer MCF-7 cells
- Clone
- 1032
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product MVP Anticorps primaire
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- Indications d'application
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1 mM EDTA buffer, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: MCF-7 or HeLa cells. Breast tumors.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Agent conservateur
- Azide free
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Date de péremption
- 24 months
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- Antigène
- MVP (Major Vault Protein (MVP))
- Autre désignation
- MVP (MVP Produits)
- Synonymes
- anticorps LRP, anticorps VAULT1, anticorps 2310009M24Rik, anticorps cb771, anticorps wu:fb52b05, anticorps wu:fc02g01, anticorps mvp, anticorps MGC145641, anticorps Tb05.45E22.810, anticorps major vault protein, anticorps major vault protein L homeolog, anticorps putative major vault protein, anticorps MVP, anticorps Mvp, anticorps mvp, anticorps mvp.L, anticorps Tc00.1047053510353.10, anticorps Tb927.5.4460, anticorps Tb10.70.0520, anticorps Tb10.70.5840, anticorps LMJF_05_0060
- Sujet
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Lung resistance-related protein (LRP), Major Vault Protein, MVP, VAULT1,Major Vault Protein (MVP)
Cellular localisation: Cytoplasmic - Poids moléculaire
- 104-110kDa
- ID gène
- 9961, 632177
- UniProt
- Q14764
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