NEFH anticorps

N° du produit ABIN6938032

Cet anticorps anti-NEFH Monoclonal Souris (Clone SPM203) (ABIN6938032) détecte spécifiquement NEFH dans WB, IHC et FACS. L’anticorps est réactif avec des échantillons de Humain, Rat, Souris, Poulet et Porc.

Aperçu rapide pour NEFH anticorps (ABIN6938032)

Antigène: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivité: Humain, Rat, Souris, Poulet, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NEFH est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: SPM203

Détail du produit anti-NEFH anticorps

Fonction: Neurofilament (NF-H) (Neuronal Marker) Antibody

Specificité: This MAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Réactivité croisée (Details): Human. Mouse. Rat. Chicken. Pig.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Triton-X 100 insoluble protein fraction of rat brain

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Western Blot(1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Brain, Neuroblastoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur NEFH

Antigène: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Autre désignation: NEFH

Sujet:

NEFH, Neurofilament H, Neurofilament Heavy Polypeptide 200 kDa, Neurofilament Triplet H Protein, NF-H, NF200

Brain, Neuroblastoma.

Poids moléculaire: 200kDa

ID gène: 4744, 198760

UniProt: P12036