NEFH & NEFL anticorps

N° du produit ABIN6938036

Cet anticorps Souris Monoclonal détecte spécifiquement NEFH & NEFL dans IHC et FACS. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Poulet et Porc.

Aperçu rapide pour NEFH & NEFL anticorps (ABIN6938036)

Antigène: NEFH & NEFL

Reactivité: Humain, Souris, Rat, Poulet, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: RT-97-NR-4

Détail du produit

Fonction: Neurofilament (H+L) (Neuronal Marker) Antibody

Specificité: This MAb reacts with a 200 kDa and 68 kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Réactivité croisée (Details): Human. Mouse. Rat. Chicken. Pig.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate.

Immunogène: Triton-X 100 insoluble proteins of rat brain (RT-97), Neurofilaments from porcine spinal cord (NR-4)

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HEK293 cells, Cerebellum, Neuroblastoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détail du antigène

Antigène: NEFH & NEFL

Sujet:

NEFH, Neurofilament H, Neurofilament Heavy Polypeptide 200 kDa, Neurofilament Triplet H Protein, NF-H, NF200. 68 kDa neurofilament protein, Light molecular weight neurofilament protein, NEFL, Neurofilament light polypeptide 68 kDa, Neurofilament light polypeptide, Neurofilament protein, light chain, Neurofilament subunit NF-L, Neurofilament triplet L protein, NF-L, NF68

HEK293 cells, Cerebellum, Neuroblastoma.

Poids moléculaire: 200kDa & 68kDa

ID gène: 4744, 198760

UniProt: P12036