NEFL anticorps

N° du produit ABIN6938039

Cet anticorps Souris Monoclonal détecte spécifiquement NEFL dans IF, FACS et IHC (f). Il présente une réactivité envers Porc.

Aperçu rapide pour NEFL anticorps (ABIN6938039)

Antigène: NEFL (Neurofilament, Light Polypeptide (NEFL))

Reactivité: Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NEFL est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: NR-4

Détail du produit anti-NEFL anticorps

Specificité: This MAb reacts with a 68 kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Réactivité croisée (Details): Human. Rat. Pig. Cow. Chicken.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Crude neurofilament preparation from porcine spinal cord

Isotype: IgG

Information d'application

Indications d'application:

Known_Application: Immunofluorescence (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HEK293 cells, Brain, Neuroblastoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur NEFL

Antigène: NEFL (Neurofilament, Light Polypeptide (NEFL))

Autre désignation: NEFL

Sujet: 68 kDa neurofilament protein, Light molecular weight neurofilament protein, NEFL, Neurofilament light polypeptide 68 kDa, Neurofilament light polypeptide, Neurofilament protein, light chain, Neurofilament subunit NF-L, Neurofilament triplet L protein, NF-L, NF68,Neurofilament (NF-L) (Neuronal Marker)
Cellular localisation: Cytoplasmic

Poids moléculaire: 68kDa

ID gène: 4747, 521461

UniProt: P07196